egfp puro Search Results


lentiviral dna plv teto hngn2 egfp puro  (Addgene inc)
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Lentiviral Dna Plv Teto Hngn2 Egfp Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell line t 2a egfp pgk puro  (Addgene inc)
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Addgene inc cell line t 2a egfp pgk puro
Cell Line T 2a Egfp Pgk Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cag cas9 t2a egfp ires puro plasmid  (Addgene inc)
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Cag Cas9 T2a Egfp Ires Puro Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pen84 plasmid  (Addgene inc)
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addgene plasmid  (Addgene inc)
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Addgene inc addgene plasmid
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral transductions pmxs puro gfp fak  (Addgene inc)
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Addgene inc retroviral transductions pmxs puro gfp fak
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
Retroviral Transductions Pmxs Puro Gfp Fak, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmscv loxp  (Addgene inc)
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Addgene inc pmscv loxp
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
Pmscv Loxp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2a egfppgk puro cassette  (Addgene inc)
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Addgene inc 2a egfppgk puro cassette
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
2a Egfppgk Puro Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oct4 egfp pgk puro vector  (Addgene inc)
93
Addgene inc oct4 egfp pgk puro vector
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
Oct4 Egfp Pgk Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control plvx egfp ires puro  (Addgene inc)
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Addgene inc control plvx egfp ires puro
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
Control Plvx Egfp Ires Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfp strep  (Addgene inc)
93
Addgene inc egfp strep
A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing <t>retroviral</t> vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.
Egfp Strep, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing retroviral vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Focal Adhesion Kinase regulates the DNA damage response and its inhibition radiosensitizes mutant KRAS lung cancer

doi: 10.1158/1078-0432.CCR-15-2603

Figure Lengend Snippet: A–B. Cell cycle analysis of H460 and H522 cells treated as indicated. The percentage of cells in each phase of the cell cycle is indicated. Note a significant increase in the percentage of G2 cells after treatment with FAKi. C. Heat maps of the genes enriched in a genome-wide expression profiling experiment, illustrating the changes in gene expression of HBEC cells expressing either pMXs-Puro-GFP or pMXs-Puro-GFP-Fak. Expression level shown is representative of +/− log (2.5) of each replicate (n = 3 samples/condition). Red signal denotes higher expression relative to the mean expression level within the group and blue signal denotes lower expression relative to the mean expression level within the group. The histogram shows Gene Ontology analysis of differentially expressed genes in enrichment analysis. D. WB analysis of H460 and H522 cells expressing the indicated shRNAs. Note upregulation of γ-H2AX in H460 cells with FAK knockdown. E. WB analysis of NSCLC cells treated with FAKi as indicated. Note upregulation of γ-H2AX in mutant, but not in wild type, KRAS NSCLC cells treated with FAKi. F. WB analysis of H460 T2.2 FAK+ and FAK− cells, note upregulation of γ-H2AX in FAK null cells. G–H. WB analysis of H460 or A549 cells (both LKB1 mutant) stably transduced with either control or with LKB1 expressing retroviral vectors (H460 or A549 + control and H460 or A549 + LKB1, respectively). Cells were treated as indicated. Note that PF-562,271 cause upregulation of γ-H2AX to a degree comparable to IR in all cell lines.

Article Snippet: Plasmids, transfections and retroviral transductions pMXs-Puro- GFP-Fak was obtained from Addgene (FAK-Plasmid #38194).

Techniques: Cell Cycle Assay, Genome Wide, Expressing, Gene Expression, Knockdown, Mutagenesis, Stable Transfection, Transduction, Control, Retroviral